LOCUS       sequence-10085-e        5649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Wang XZ, Ron D
  TITLE     Stress-induced phosphorylation and activation of the transcription 
            factor CHOP (GADD153) by p38 MAP Kinase.
  JOURNAL   Science. 1996 May 31. 272(5266):1347-9.
  PUBMED    8650547
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1996 May 31. 272(5266):1347-9."
COMMENT     Sequence Label: 9E10.ChWT.Gal4.pCDNA1/AMP
FEATURES             Location/Qualifiers
     source          1..5649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(603..1061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(740..761)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     955..974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(1186..1204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1272..1376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1377..1445,1446..2237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1595..1614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        2310..2689
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2690..2893
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2844..2864
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2938..2957
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2938..2956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2994..3023
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3423..3857
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="LLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRS
                     PLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDA
                     VTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(3921..3939)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3922..3939)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          4023..4088
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4218..4238
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4510..4644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4547..4566)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4601..4620
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5490..5625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
       61 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      361 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
      601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      661 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      721 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      781 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
      841 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      961 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1021 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     1081 gaccgtataa cgtgctttcc tcgttggaat cagagcggga gctaaacagg aggccgatta
     1141 aagggatttt agacaggaac ggtacgccag ctggattacc aaagggcctc gtgatacgcc
     1201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     1621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1921 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2281 aacttcattt ttaatttctc tagcgcgttg acattgatta ttgactagtt attaatagta
     2341 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2401 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2461 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
     2521 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2581 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2641 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2701 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2761 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2821 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2881 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     2941 tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccgcc atggagcaaa
     3001 agctgatttc tgaggaggat ctggcagctg agtccctgcc tttcaccttg gagacggtgt
     3061 ccagctggga gctggaagcc tggtatgagg atctgcagga ggtcctgtcc tcagatgaaa
     3121 ttgggggcac ctatatctca tccccaggaa acgaagagga agaatcaaaa accttcacta
     3181 ctcttgaccc tgcgtcccta gcttggctga cagaggagcc agggccaaca gaggtcacac
     3241 gcacatccca aagccctcgc tctccagatt ccagtcagag ttctatggcc caggaggaag
     3301 aggaggaaga gcaaggaaga actaggaaac ggaaacagag tggtcagtgc ccagcccggc
     3361 ctgggaagca acgcatgaag gagaaggagc aggagaacga gcggaaagtg gcacagctag
     3421 cgctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag ctcaagtgct
     3481 ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc
     3541 ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa tcaaggctag
     3601 aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac atgattttga
     3661 aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta caagataatg
     3721 tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg cctctaacat
     3781 tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac aaaggtcaaa
     3841 gacagttgac tgtatcgccg gaattctgca gatatccatc acactggcgg ccgctcgagc
     3901 atgcatctag agggccctat tctatagtgt cacctaaatg ctagaggatc tttgtgaagg
     3961 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     4021 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     4081 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     4141 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     4201 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     4261 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     4321 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     4381 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     4441 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4501 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4621 aatgtatctt atcatgtctg gatcatcccg ccatggtatc aacgccatat ttctatttac
     4681 agtagggacc tcttcgttgt gtaggtaccg ctgtattcct agggaaatag tagaggcacc
     4741 ttgaactgtc tgcatcagcc atatagcccc cgctgttcga cttacaaaca caggcacagt
     4801 actgacaaac ccatacacct cctctgaaat acccatagtt gctagggctg tctccgaact
     4861 cattacaccc tccaaagtca gagctgtaat ttcgccatca agggcagcga gggcttctcc
     4921 agataaaata gcttctgccg agagtcccgt aagggtagac acttcagcta atccctcgat
     4981 gaggtctact agaatagtca gtgcggctcc cattttgaaa attcacttac ttgatcagct
     5041 tcagaagatg gcggagggcc tccaacacag taattttcct cccgactctt aaaatagaaa
     5101 atgtcaagtc agttaagcag gaagtggact aactgacgca gctggccgtg cgacatcctc
     5161 ttttaattag ttgctaggca acgccctcca gagggcgtgt ggttttgcaa gaggaagcaa
     5221 aagcctctcc acccaggcct agaatgtttc cacccaatca ttactatgac aacagctgtt
     5281 ttttttagta ttaagcagag gccggggacc cctgggcccg cttactctgg agaaaaagaa
     5341 gagaggcatt gtagaggctt ccagaggcaa cttgtcaaaa caggactgct tctatttctg
     5401 tcacactgtc tggccctgtc acaaggtcca gcacctccat acccccttta ataagcagtt
     5461 tgggaacggg tgcgggtctt actccgccca tcccgcccct aactccgccc agttccgccc
     5521 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5581 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5641 agctaattc
//
