LOCUS       sequence-100868-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Localization, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             81..101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             105..125
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    255..376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(292..311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     346..365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(520..541)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     732..751
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga gctgatccaa
       61 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa
      121 aggtaggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
      181 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      241 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      301 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      361 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
      421 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
      481 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
      541 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
      601 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
      661 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
      721 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
      781 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gg
//
