LOCUS       sequence-100872-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. Encodes membrane bound dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-26"; volume: "10(5):e0127822. doi"; pages: " 
            10.1371/journal.pone.0127822. eCollection 2015"
COMMENT     Sequence Label: pMTB-Multibow-mR
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      148..157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(320..338)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     588..607
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttcgtatagg atactttata cgaagttatt gatcaattca cagccaccat gctgtgctgc
       61 atcagaagaa ctaaaccggt tgagaagaat gaagaggccg atcaggagct gcagtcgacg
      121 gtaccgcggg cccgggatcc accggtcgcc accatgggag cgccagctgg aggtgcaggt
      181 gcagcaatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
      241 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      301 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
      361 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
      421 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      481 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
      541 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      601 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
      661 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
      721 aagaccatct acatggccaa gaagcccgtg caactg
//
