LOCUS       sequence-100879-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid mediates polycistronic expression of 4 genes: Ngn3, 
            Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic 
            acinar cells to induced beta-like cells in vivo. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, 
            Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y,
            Zeng S, Gifford D, Meissner A, Weir G, Zhou Q
  TITLE     Long-term persistence and development of induced pancreatic beta 
            cells generated by lineage conversion of acinar cells.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub
            2014 Nov 17.
  PUBMED    25402613
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3082"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1223-30"
COMMENT     Sequence Label: pAd-M3cherry
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..301
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 agaggaagat aagaaacgta gtagcgggac cccgagtggg ggcggtgggg gcgaagagcc
       61 ggaacaagat tgtgcggtga cctcgggcga ggagctgctg gcagtgccac cgctgccacc
      121 tcccggaggt gccgtgcccc caggcgtccc agctgcagtc cgggagggcc tactgccttc
      181 gggccttagc gtgtcgccac agccctccag catcgcgcca ctgcgaccgc aggaaccccg
      241 gggatccgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
      301 tatcgatatg gccgcggagc tggcgatggg cgcagagctg cccagcagcc cactggccat
      361 cgagtacgtc aacgacttcg acctgatgaa gttcgaggtg aagaaggagc cgcccgaggc
      421 cgagcgcttc tgccaccgcc tgccgcccgg ctcgctgtcc tcgacgcccc tcagcacgcc
      481 ctgctcctcg gtgccctctt cgcccagctt ctgcgcaccc agcccgggca caggcggcgg
      541 cgcgggcggc gggggcagcg cggctcaggc cgggggcgcc ccggggccgc cgagtggagg
      601 ccccggcact gtcgggggcg cctcaggaaa agcggtgctg gaggatctgt actggatgag
      661 cgggtaccag caccacctga accccgaggc gctcaacctg acgccggagg acgcggtgga
      721 ggcgctcatc ggcagcggcc accacggcgc gcaccacggc gcgcatcacc cggcggctgc
      781 tgcggcctat gaggccttcc ggggtcagag cttc
//
