LOCUS       sequence-100882-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-ER-5
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..797
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 ccgtcagatc cgctagcgcc accatgctgc tatccgtgcc gttgctgctc ggcctcctcg
       61 gcctggccgt cgccgcaccg gtcgccacca tggtgagcaa gggcgaggag aataacatgg
      121 ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg
      181 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggctttcag accgctaagc
      241 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcatttca
      301 cctacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttcaagctgt
      361 ccttccccga gggcttcaag tgggagcgcg tgatgaacta cgaggacggc ggcgtggtga
      421 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      481 gcaccaactt cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggcct
      541 cctccgagcg gatgtacccc gaggacggtg ccctgaaggg caagatcaag atgaggctga
      601 agctgaagga cggcggccac tacacctccg aggtcaagac cacctacaag gccaagaagc
      661 ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcacc tcccacaacg
      721 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
      781 tggacgagct gtacaagtcc ggaa
//
