LOCUS       sequence-100884-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of KIN28 under GAL1 promoter in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Bieganowski P, Garrison PN, Hodawadekar SC, Faye G, Barnes LD, 
            Brenner C
  TITLE     Adenosine monophosphoramidase activity of Hint and Hnt1 supports 
            function of Kin28, Ccl1, and Tfb3.
  JOURNAL   J Biol Chem. 2002 Mar 29;277(13):10852-60. Epub 2002 Jan 22.
  PUBMED    11805111
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-03-29"; volume: "277(13)"; pages: "10852-60. 
            Epub 2002 Jan 22"
COMMENT     Sequence Label: pB194
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..460
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    19..136
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     431..454
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 aaagctggag ctctagtacg gattagaagc cgccgagcgg gtgacagccc tccgaaggaa
       61 gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc
      121 gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg ttatgaagag
      181 gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg aacgaatcaa attaacaacc
      241 ataggatgat aatgcgatta gttttttagc cttatttctg gggtaattaa tcagcgaagc
      301 gatgattttt gatctattaa cagatatata aatgcaaaaa ctgcataacc actttaacta
      361 atactttcaa cattttcggt ttgtattact tcttattcaa atgtaataaa agtatcaaca
      421 aaaaattgtt aatatacctc tatactttaa cgtcaaggag aaaaaacccc ggattctaga
      481 actagtgatt catcatatga aagtgaatat ggagtacaca aaggaaaaga aagttggtga
      541 gggtacttat gcggttgttt acttgggttg tcaacactct actggaagaa agattgctat
      601 taaggagatc aaaacatccg aatttaaaga tggtttagat atgtcagcta tccgtgaagt
      661 taagtacctc caagaaatgc agcatccgaa cgtcatagaa ctaatagaca tatttatggc
      721 ttatgataat ttaaacctcg ttctggagtt cctaccaact gatctagagg tggtaataaa
      781 agacaaatca atactgttta caccagcaga tattaaggca tggatgctta tgactttgag
      841 gggcgtgtat cattgccaca gaaatttcat tttgc
//
