LOCUS       sequence-100886-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knock-in donor vector to insert Exon 44 in front of Exon 45 of human
            DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette 
            flanked by loxP sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka 
            M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A
  TITLE     Precise Correction of the Dystrophin Gene in Duchenne Muscular 
            Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and 
            CRISPR-Cas9.
  JOURNAL   Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013.
  PUBMED    25434822
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013."
COMMENT     Sequence Label: pENTR-DMD-Donor
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcccgggcc accatgaaga aacctgaact gacagcaact tctgttgaga agtttctcat
       61 tgaaaaattt gattctgttt ctgatctcat gcagctgtct gaaggtgaag aaagcagagc
      121 cttttctttt gatgttggag gaagaggtta tgttctgagg gtcaattctt gtgctgatgg
      181 tttttacaaa gacagatatg tttacagaca ctttgcctct gctgctctgc caattccaga
      241 agttctggac attggagaat tttctgaatc tctcacctac tgcatcagca gaagagcaca
      301 aggagtcact ctccaggatc tccctgaaac tgagctgcca gctgttctgc aacctgttgc
      361 tgaagcaatg gatgccattg cagcagctga tctgagccaa acctctggat ttggtccttt
      421 tggtccccaa ggcattggtc agtacaccac ttggagggat ttcatttgtg ccattgctga
      481 tcctcatgtc tatcactggc agactgtgat ggatgacaca gtttctgctt ctgttgctca
      541 ggcactggat gaactcatgc tgtgggcaga agattgtcct gaagtcagac acctggtcca
      601 tgctgatttt ggaagcaaca atgttctgac agacaatggc agaatcactg cagtcattga
      661 ctggtctgaa gccatgtttg gagattctca atatgaggtt gccaacat
//
