LOCUS       sequence-10089-e        1271 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Kobashigawa Y, Kumeta H, Ogura K, Inagaki F
  TITLE     Attachment of an NMR-invisible solubility enhancement tag using a 
            sortase-mediated protein ligation method.
  JOURNAL   J Biomol NMR. 2009 Mar . 43(3):145-50.
  PUBMED    19140010
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomol 
            NMR. 2009 Mar . 43(3):145-50."
COMMENT     Sequence Label: pGBMCS-SortA
FEATURES             Location/Qualifiers
     source          1..1271
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..168
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             628..645
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             657..818
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
ORIGIN
        1 atggagtaca aactgatcct gaacggtaaa accctgaaag gtgaaaccac caccgaagct
       61 gttgacgctg ctaccgcgga aaaagttttc aaacagtacg ctaacgacaa cggtgttgac
      121 ggtgaatgga cctacgacga cgctaccaaa accttcacgg taaccgaaca tatgggatcc
      181 caagctaaac ctcaaattcc gaaagataaa tcaaaagtgg caggctatat tgaaattcca
      241 gatgctgata ttaaagaacc agtatatcca ggaccagcaa cacctgaaca attaaataga
      301 ggtgtaagct ttgcagaaga aaatgaatca ctagatgatc aaaatatttc aattgcagga
      361 cacactttca ttgaccgtcc gaactatcaa tttacaaatc ttaaagcagc caaaaaaggt
      421 agtatggtgt actttaaagt tggtaatgaa acacgtaagt ataaaatgac aagtataaga
      481 gatgttaagc caacagatgt agaagttcta gatgaacaaa aaggtaaaga taaacaatta
      541 acattaatta cttgtgatga ttacaatgaa aagacaggcg tttgggaaaa acgtaaaatc
      601 tttgtagcta cagaagtcaa actcgagcac caccaccacc accactgacc atggagtaca
      661 aactgatcct gaacggtaaa accctgaaag gtgaaaccac caccgaagct gttgacgctg
      721 ctaccgcgga aaaagttttc aaacagtacg ctaacgacaa cggtgttgac ggtgaatgga
      781 cctacgacga cgctaccaaa accttcacgg taaccgaaca tatgggatcc caagctaaac
      841 ctcaaattcc gaaagataaa tcaaaagtgg caggctatat tgaaattcca gatgctgata
      901 ttaaagaacc agtatatcca ggaccagcaa cacctgaaca attaaataga ggtgtaagct
      961 ttgcagaaga aaatgaatca ctagatgatc aaaatatttc aattgcagga cacactttca
     1021 ttgaccgtcc gaactatcaa tttacaaatc ttaaagcagc caaaaaaggt agtatggtgt
     1081 actttaaagt tggtaatgaa acacgtaagt ataaaatgac aagtataaga gatgttaagc
     1141 caacagatgt agaagttcta gatgaacaaa aaggtaaaga taaacaatta acattaatta
     1201 cttgtgatga ttacaatgaa aagacaggcg tttgggaaaa acgtaaaatc tttgtagcta
     1261 cagaagtcaa a
//
