LOCUS       sequence-100890-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Hnt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Bieganowski P, Garrison PN, Hodawadekar SC, Faye G, Barnes LD, 
            Brenner C
  TITLE     Adenosine monophosphoramidase activity of Hint and Hnt1 supports 
            function of Kin28, Ccl1, and Tfb3.
  JOURNAL   J Biol Chem. 2002 Mar 29;277(13):10852-60. Epub 2002 Jan 22.
  PUBMED    11805111
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-03-29"; volume: "277(13)"; pages: "10852-60. 
            Epub 2002 Jan 22"
COMMENT     Sequence Label: pB24
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..46)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     RBS             561..583
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    598..622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(622..641)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(623..641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(725..744)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcagccgga tccgcgaccc atttgctgtc caccagtcat gctagcaggc ctgatcgatc
       61 catgggtacc ctcgagctaa tcggagcctt ctagtttggc aagcaattcc ttgtgtagct
      121 tgcccaactt atcgaagtcc gtttcttggg ctggccaccc tacaatcaaa ccacttttct
      181 catctctctt aggaatcaaa tggaagtgga cgtggtcgac ttcttgatgc gcaattttac
      241 cattattctg caacacatta taagtgtcca acttcattgc cttggccagt ctcttggcaa
      301 tcggcatagc atcggtaagg aattcgtccg ggatgtcatg caacttcgca ccatggtact
      361 taggaatgat taaagcatga ccttcagcag taggttggat gtccaagaaa gcatacgagt
      421 actttgtttc aatcaatttg aaggatggaa tttcgctttt aataatcttg caaaaaatac
      481 aggcagcatc aagcgtagca ggagcagaca tttttgttgt tgttaggtac ggtgccgata
      541 tcaatggctc catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga
      601 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      661 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      721 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      781 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
      841 atctccttgc atgcaccatt ccttgcggcg gcggtgctca
//
