LOCUS       sequence-100892-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses halo tagged human Keap1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Fang X, Fu Y, Long MJ, Haegele JA, Ge EJ, Parvez S, Aye Y
  TITLE     Temporally controlled targeting of 4-hydroxynonenal to specific 
            proteins in living cells.
  JOURNAL   J Am Chem Soc. 2013 Oct 2;135(39):14496-9. doi: 10.1021/ja405400k. 
            Epub 2013 Sep 18.
  PUBMED    24015839
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja405400k";
            journalName: "J Am Chem Soc"; date: "2013-10-2- 2"; volume: "135"; 
            issue: "39"; pages: "14496-9"
COMMENT     Sequence Label: pMIR-DsRed-IRES-His-Halo-Tev-Keap1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             180..197
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag
       61 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
      121 atgataatat ggccacaacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      181 tggtgcctcg tggtagccat atggcagaaa tcggtactgg ctttccattc gacccccatt
      241 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
      301 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
      361 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
      421 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
      481 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
      541 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
      601 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
      661 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
      721 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
      781 tgaaccctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
      841 agccagcgaa catcgtcgcg ctggtcgaag
//
