LOCUS       sequence-100894-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN vector with G418 resistance marker for single Gateway insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p41Neo 1-F GW
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(265..281)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(266..282)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      284..531
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(300..318)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(549..568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(550..568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(578..595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(578..594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(587..609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(822..841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgggct
      241 gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagtcatgta attagttatg
      301 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
      361 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
      421 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
      481 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtac
      541 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
      601 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      661 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      721 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      781 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      841 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
//
