LOCUS       sequence-100897-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by PBAD. The C-terminal fragment has the point 
            mutation N748D. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-N748D
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(67..94)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gagccaaaat aataaaaaag caggattaat aatctggctt tttatattct ctctctagta
       61 tataaacgca gaaaggccca cccgaaggtg agccagtgtg atccaagcca gctcgatatc
      121 cgtcggcttg aacgaattgt tagacattac ttgtagacgt gccctacgcg cttgttgagt
      181 tgttcctcaa cgtttttctt gaagtgctta gcttcaaggt cacggatacg accgaagcga
      241 gcctcgtcct caatggcccg accgattgcg cttgctacag cctgaacggt tgtattgtca
      301 gcactggtta ggcaagccag agtggtctta atggtgatgt acgctacggc ttccggcttg
      361 atttcttgca ggaactggaa ggctgtcggg cgcttgccgc gcttagcttt cacttcctca
      421 aaccagtcgt tgatgcgtgc aatcatctta gggagtaggg tagtgatgag aggcttggcg
      481 gcagcgttat ccgcaacctc accagcttta agttgacgct caaacatctt gcggaagcgt
      541 gcttcaccca tctcgtaaga ctcatgctca agggccaact gttcgcgagc taaacgctca
      601 ccgtaatggt cagccagagt gttgaacggg atagcagcca gttcgatgtc agagaagtcg
      661 ttcttagcga tgttaatcgt gttcatatta gaaaaagaat gttattaagt aagtctttct
      721 agagctcgct agccc
//
