LOCUS       sequence-100900-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by PBAD. The C-terminal fragment has the point 
            mutation R756S. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-R756S
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(44..71)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gattaataat ctggcttttt atattctctc tctagtatat aaacgcagaa aggcccaccc
       61 gaaggtgagc cagtgtgatc caagccagct cgatatccgt cggcttgaac gaattgttag
      121 acattacttg tagacgtgcc ctacgcgctt gttgagttgt tcctcaacgt ttttcttgaa
      181 gtgcttagct tcaaggtcac ggatacgacc gaagcgagcc tcgtcctcaa tggcccgacc
      241 gattgcgctt gctacagcct gaacggttgt attgtcagca ctggttaggc aagccagagt
      301 ggtcttaatg gtgatgtacg ctacggcttc cggcttgatt tcttgcagga actggaaggc
      361 tgtcgggcgc ttgccgcgct tagctttcac ttcctcaaac cagtcgttga tgcgtgcaat
      421 catcttaggg agtagggtag tgatgagagg cttggcggca gcgttatccg caacctcacc
      481 agctttaagt tgacgctcaa acatcttgcg gaagcgtgct tcacccatct cgtaagactc
      541 atgctcaagg gccaactgtt cgcgagctaa acgctcaccg taatggtcag ccagagtgtt
      601 gaacgggata gcagccagtt cgatgtcaga gaagtcgttc ttagcgatgt taatcgtgtt
      661 catattagaa aaagaatgtt attaagtaag tctttctaga gctcgctagc cc
//
