LOCUS       sequence-100909-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    27..79
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      583..592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(619..718)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(648..668)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
       61 ttcgaagctt gagctcgaga gtgctcccgc cactgccctc cgagggcacc gcggccggac
      121 tggcaggcgc cgccggggtg cggggggcag cgtttcgcgc gcgggcgcgg gccacgccgc
      181 tgcaggacac cacgccgtgg cgccggcgga agcggtccag ccagccgttg gaggcggtga
      241 agtcgtccat gcccagctcc tcggctatgc gcagcgcctt ctccttgagg atgatgccct
      301 tgaccggcag gccggcggcg cggatctgct ggaaccaggc gatgagcaag ccctcgagct
      361 tgtcgtaggg agacagcttg ttggtcttgc ggcaggtgga ggccaccccg tacttgcgct
      421 ccgacgccag gatggcgcgc ttgttcttca ggatcgtgct cagcgtggac ggcgggatgt
      481 tgaagcgccg cgcgatctcg cccttgcgta ggtccgggtt ctcctccacc tcctggatga
      541 tccgcgactt ctcccggaac gtcagctgcc gccgcttggg gcccatggtg gctagcggat
      601 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
      661 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
      721 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatccc
//
