LOCUS       sequence-100910-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 targeting vector, Cre-dependent tdtomato expression. See 
            article for more colors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, 
            Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H
  TITLE     A robust and high-throughput Cre reporting and characterization 
            system for the whole mouse brain.
  JOURNAL   Nat Neurosci. 2010 Jan . 13(1):133-40.
  PUBMED    20023653
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2010 Jan . 13(1):133-40."
COMMENT     Sequence Label: Ai9
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(92..115)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(290..309)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     559..577
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      707..716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(791..824)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgccgctgc cggtgctgcc ggtgccatgc cccaggaaca ggtggtggcg gccctcggag
       61 cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg
      121 tccacgtagt agtagccggg cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac
      181 tccaccaggt agtggccgcc gtccttcagc ttcagggcct ggtggatctc gcccttcagc
      241 acgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatggt cttcttctgc
      301 attacggggc cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg
      361 ccgtcctgca gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc gaagttcatc
      421 acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtaatcggg gatgtcggcg
      481 gggtgcttca cgtacgcctt ggagccgtac atgaactggg gggacaggat gtcccaggcg
      541 aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      601 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcatggagcc ctccatgcgc
      661 accttgaagc gcatgaactc tttgatgacc tcctcgccct tgctcaccat ggtggcggga
      721 tcgtgttgca cttaacgcgt acaaggccgg ccgaattcga tctagcttgg gctgcaggtc
      781 gagggaccta ataacttcgt atagcataca ttatacgaag ttatattaag ggttccggat
      841 cagcttga
//
