LOCUS       sequence-100913-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaEdN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-HY43
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             80..102
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             674..691
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(731..748)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      901..987
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggggtgcgg atctacctga cgctttttat cgcactctct actgtttctc catacccgtt
       61 tttttgggct agaaataatt ttgtttaact ttaagaagga gatatacata tggaatatgg
      121 attattaccg attggtaaaa ttgtagaaaa gcgcatcgaa tgtactgttt atagcgttga
      181 taataatgga aatatttata cacaacctgt agcacaatgg cacgatcgcg gagaacaaga
      241 ggtgtttgag tattgtttgg aagatggttc attgattcgg gcaacaaaag accataagtt
      301 tatgactgtt gatggtcaaa tgttgccaat tgatgaaata tttgaacgtg aattggattt
      361 gatgcgggtt gataatttgc cgaatatcaa aatagccaca cgtaaatatt taggcaaaca
      421 aaatgtctat gacattggag ttgagcgcga ccataatttt gcactcaaaa atggcttcat
      481 agcttctaat tgtttcaatg gtacccagta caaacttatc ctgaacggta aaaccctgaa
      541 aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt tcaaacagta
      601 cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca aaacctacac
      661 cgtaacggaa ttccatcatc atcatcatca ttaagcttgg ctgttttggc ggatgagaga
      721 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
      781 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
      841 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
      901 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      961 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcac
     1021 gcccggaagg ggtgggcggg gcaggacgcc cgccataaac tgccaggcat caaatttaag
     1081 cagagccatc ctgacggatg gccttttttg cgtttctaca aactctttgt aatt
//
