LOCUS       sequence-100915-        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NpuDnaB(delta283)dN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-111
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             79..101
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             697..714
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(754..771)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      924..1010
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggggtcggat ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt
       61 ttttgggcta gaaataattt tgtttaactt taagaaggag atatacatat gggatccgga
      121 ctgcaagtgc caattaagga attagtaggt aaatctggtt ttgcagtttg ggcattgaac
      181 gaagctacaa tgcagctaga aaaggcaatt gttagcaatg ctttttcgac tggtataaag
      241 cctctgttta ctttgacaac tcgattgggg cggaaaatcc gtgccacagg taatcacaag
      301 tttttaacaa ttaatggctg gaaaagactt gatgaattaa ctcctaaaga acatctcgct
      361 ttaccaagaa atctccccag ttctggtact cttgccaaaa gtgatattta ttgggatgaa
      421 atagtttcaa ttgaatatag tggtgaggaa gaagtgtttg acctcaccgt tcctggtttg
      481 cataattttg ttgcaaataa tattattgtt cacaattcca ttggtaccca gtacaaactt
      541 atcctgaacg gtaaaaccct gaaaggtgaa accaccaccg aagctgttga cgctgctacc
      601 gcggaaaaag ttttcaaaca gtacgctaac gacaacggtg ttgacggtga atggacctac
      661 gacgacgcta ccaaaaccta caccgtaacg gaattccatc atcatcatca tcattaagct
      721 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
      781 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
      841 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
      901 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      961 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     1021 cggatttgac gttgcgagca cgcccgggaa gggtggcggg caggacgccc gccataaact
     1081 gccaggcatc aaattaagca gaagccatcc tgacg
//
