LOCUS       sequence-100919-        5329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TvoVMAdN11 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSABAD14-98
FEATURES             Location/Qualifiers
     source          1..5329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             713..730
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(770..787)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      939..1025
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1117..1144
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1162..1253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1254..1322,1323..2114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1472..1491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2156..2611
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2243..2262)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2453..2474
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2722..3310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3211..3230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3464..3481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3496..3636
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3562..3581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3722..3744
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3883..3902
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(4085..4963)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4990..5274
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5197..5216
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             5301..5323
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatggatggc aagacaataa aaataaagga tctatacagt tctgagagaa aaaaagaaga
       61 taacattgtt gaggctggtt cgggagaaga gataatacat ctaaaagatc ccattcaaat
      121 atattcttat gtggacggga ccatagtcag gagcagatca agacttctat acaagggcaa
      181 gagctcttat cttgtgagga tagaaactat tggcggaaga tcggtaagcg ttacaccagt
      241 tcacaaactc tttgtcctta cggaaaaggg tatcgaagag gttatggcct ccaacctaaa
      301 ggtaggcgac atgattgctg ctgtagcaga aagcgaatct gaagcaagag actgcggaat
      361 gagcgaggaa tgcgtgatgg aagcagaagt ttatacgtca cttgaagcga cattcgatag
      421 agtaaagtct atagcgtacg agaagggtga ttttgatgta tacgatcttt ccgtaccaga
      481 atacggcagg aactttatag gcggagaagg acttctcgta cttcacaaca cggttatagg
      541 tacccagtac aaacttatcc tgaacggtaa aaccctgaaa ggtgaaacca ccaccgaagc
      601 tgttgacgct gctaccgcgg aaaaagtttt caaacagtac gctaacgaca acggtgttga
      661 cggtgaatgg acctacgacg acgctaccaa aacctacacc gtaacggaat tccatcatca
      721 tcatcatcat taagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga
      781 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg
      841 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg
      901 tggggtcccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      961 gcaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1021 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     1081 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     1141 tttttgcgtt tctacaaact ctttgtttat ttttctaaat acattcaaat atgtatccgc
     1201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1261 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1321 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1381 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1441 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1501 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1561 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1621 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1681 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1741 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     1801 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1861 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1921 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1981 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2041 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2101 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttacgcg
     2161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2341 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     2401 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2461 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     2521 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2581 aattttaaca aaatattaac gtttacaatt taaaaggatc taggtgaaga tcctttttga
     2641 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2701 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2761 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2821 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2881 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2941 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3001 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3061 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     3121 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3181 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3241 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3301 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3361 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3421 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3481 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3541 ccggatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     3601 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     3661 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3721 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa
     3781 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     3841 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     3901 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     3961 gatctaattc tcatgtttga cagcttatca tcgatgcata atgtgcctgt caaatggacg
     4021 aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta
     4081 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     4141 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     4201 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     4261 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     4321 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     4381 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     4441 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     4501 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     4561 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     4621 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     4681 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     4741 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     4801 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     4861 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     4921 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
     4981 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
     5041 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
     5101 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
     5161 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
     5221 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
     5281 ttttttgggc tagaaataat tttgtttaac tttaagaagg agatataca
//
