LOCUS       sequence-100922-         546 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector for bi-cistronic expression of EGFP
            and the gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Lou E, Fujisawa S, Morozov A, Barlas A, Romin Y, Dogan Y, Gholami S,
            Moreira AL, Manova-Todorova K, Moore MA
  TITLE     Tunneling nanotubes provide a unique conduit for intercellular 
            transfer of cellular contents in human malignant pleural 
            mesothelioma.
  JOURNAL   PLoS One. 2012;7(3):e33093. doi: 10.1371/journal.pone.0033093. Epub 
            2012 Mar 9.
  PUBMED    22427958
REFERENCE   2  (bases 1 to 546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e33093. doi"; pages: " 
            10.1371/journal.pone.0033093. Epub 2012 Mar 9"
COMMENT     Sequence Label: pUltra
FEATURES             Location/Qualifiers
     source          1..546
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(204..223)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gctctcgccc ttgctcacca tggtggcgac cggtggatcg gacctgcagc ccaagcttcg
       61 tctaacaaaa aagccaaaaa cggccagaat ttagcggaca atttactagt ctaacactga
      121 aaattacata ttgacccaaa tgattacatt tcaaaaggtg cctaaaaaac ttcacaaaac
      181 acactcgcca accccgagcg catagttcaa aaccggagct tcagctactt aagaagatag
      241 gtacataaaa ccgaccaaag aaactgacgc ctcacttatc cctcccctca ccagaggtcc
      301 ggcgcctgtc gattcaggag agcctaccct aggcccgaac cctgcgtcct gcgacggaga
      361 aaagcctacc gcacacctac cggcaggtgg ccccaccctg cattataagc caacagaacg
      421 ggtgacgtca cgacacgacg agggcgcgcg ctcccaaagg tacgggtgca ctgcccaacg
      481 gcaccgccat aactgccgcc cccgcaacag acgacaaacc gagttctcca gtcagtgaca
      541 aacttc
//
