LOCUS       sequence-100931-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon with inducible SfiI cloning site for GOI (contains 
            firefly luciferase) and constitutive expression of GFP, rtTA and 
            neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Kowarz E, Loescher D, Marschalek R
  TITLE     Optimized Sleeping Beauty transposons rapidly generate stable 
            transgenic cell lines.
  JOURNAL   Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821.
  PUBMED    25650551
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Feb 4. doi: 10.1002/biot.201400821."
COMMENT     Sequence Label: pSBtet-GN
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcttttgcct tatacctact ttccgtacca cttcctaccc tcgaaaggcc tctgaggcca
       61 ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
      121 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
      181 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
      241 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
      301 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
      361 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
      421 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
      481 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
      541 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
      601 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
      661 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
      721 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
      781 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
      841 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
      901 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
      961 tcatcgacaa gtacgaccta agcaacttgc acg
//
