LOCUS       sequence-100936-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes SNAP-AP fusion for tagging endogenous target for 
            SNAP-SiMPull.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Rodgers ML, Paulson J, Hoskins AA
  TITLE     Rapid isolation and single-molecule analysis of ribonucleoproteins 
            from cell lysate by SNAP-SiMPull.
  JOURNAL   RNA. 2015 May;21(5):1031-41. doi: 10.1261/rna.047845.114. Epub 2015 
            Mar 24.
  PUBMED    25805862
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.047845.114"; journalName: "RNA"; date: "2015-05"; 
            volume: "21"; issue: "5"; pages: "1031-41"
COMMENT     Sequence Label: pSNAPf-AP
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     23..40
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             67..612
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             631..675
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 ccttatgtat catacacata cgatttaggt gacactatag aacgcggccg ccagctgaag
       61 cttcatatgg acaaagactg cgaaatgaag cgcaccaccc tggatagccc tctgggcaag
      121 ctggaactgt ctgggtgcga acagggcctg caccgtatca tcttcctggg caaaggaaca
      181 tctgccgccg acgccgtgga agtgcctgcc ccagccgccg tgctgggcgg accagagcca
      241 ctgatgcagg ccaccgcctg gctcaacgcc tactttcacc agcctgaggc catcgaggag
      301 ttccctgtgc cagccctgca ccacccagtg ttccagcagg agagctttac ccgccaggtg
      361 ctgtggaaac tgctgaaagt ggtgaagttc ggagaggtca tcagctacag ccacctggcc
      421 gccctggccg gcaatcccgc cgccaccgcc gccgtgaaaa ccgccctgag cggaaatccc
      481 gtgcccattc tgatcccctg ccaccgggtg gtgcagggcg acctggacgt ggggggctac
      541 gagggcgggc tcgccgtgaa agagtggctg ctggcccacg agggccacag actgggcaag
      601 cctgggctgg gtgtcgacgg cggttcagcg ggcctgaacg acatcttcga ggcccagaag
      661 atcgagtggc acgagtaagg atccccgggt taattaaggc gcgccagatc tgtttagctt
      721 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      781 agtcttgacg tgcgcagctc aggggcatga tgtgact
//
