LOCUS       sequence-100960-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The codon-optimized Cas9 nuclease under the control of the 
            Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is 
            low copy number..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, 
            Wildonger J, O'Connor-Giles KM
  TITLE     Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 
            nuclease.
  JOURNAL   Genetics. 2013 May 24.
  PUBMED    23709638
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 May 24."
COMMENT     Sequence Label: pHsp70-Cas9
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcttacgat acaattagta tctaatatac atgggttcat tctacattct atattagtga
       61 tgatttcttt agctagtaat acattttaat tatattcggc tttgatgatt ttctgatttt
      121 ttccgaacgg attttcgtag accctttcga tctcataatg gctcatttta ttgcgatgga
      181 cggtcaggag agctccactt ttgaatttct gttcgcagac accgcatttg tagcacatag
      241 ccgggacatc cggtttgggg agattttcca gtctctgttg caattggttt tcgggaatgc
      301 gttgcaggcg catacgctct atatcctccg aacggcgctg gttgacccta gcatttacat
      361 aaggatcagc agcaaaattt gcctctactt cattgcccgg aatcacagca atcagatgtc
      421 cctttcggtt acgatggata ttcaggtgcg aaccgcacac aaagctctcg ccgcacactc
      481 cacactgata tggtcgctcg cccgtgtggc gccgcatatg gatcttaagg tcgttggact
      541 gcacaaagct cttgctgcac attttgcagg agtacggcct ttgacccgtg tgcaatcgca
      601 tgtgtcgcgc cagcttgttc tgcgaaataa actaacggga attcctgcag cccgggggat
      661 ccgcggccgc ccggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
      721 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
      781 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
      841 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
      901 gcgataccgt aaagcacgag gaagc
//
