LOCUS       sequence-100969-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Phagemid expressing streptomycin/spectinomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Firnberg E, Ostermeier M
  TITLE     PFunkel: efficient, expansive, user-defined mutagenesis.
  JOURNAL   PLoS One. 2012;7(12):e52031. doi: 10.1371/journal.pone.0052031. Epub
            2012 Dec 17.
  PUBMED    23284860
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(12):e52031. doi"; pages: " 
            10.1371/journal.pone.0052031. Epub 2012 Dec 17"
COMMENT     Sequence Label: pSkunk3-BLA
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(173..275)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(667..684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
       61 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
      121 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc attttagctt
      181 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
      241 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
      301 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
      361 ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt
      421 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
      481 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag
      541 cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg
      601 tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc
      661 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
      721 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
      781 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacg
//
