LOCUS       sequence-100973-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc-inducible Beta recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-14"; volume: "346(6211):1256272. doi"; pages: " 
            10.1126/science.1256272"
COMMENT     Sequence Label: pFF145
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..786
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
ORIGIN
        1 atgagtactg cactcgcaac gctggctggg aagctggctg aacgtgtcgg catggattct
       61 gtcgacccac aggaactgat caccactctt cgccagacgg catttaaagg tgatgccagc
      121 gatgcgcagt tcatcgcatt actgatcgtt gccaaccagt acggccttaa tccgtggacg
      181 aaagaaattt acgcctttcc tgataagcag aatggcatcg ttccggtggt gggcgttgat
      241 ggctggtccc gcatcatcaa tgaaaaccag cagtttgatg gcatggactt tgagcaggac
      301 aatgaatcct gtacatgccg gatttaccgc aaggaccgta atcatccgat ctgcgttacc
      361 gaatggatgg atgaatgccg ccgcgaacca ttcaaaactc gcgaaggcag agaaatcacg
      421 gggccgtggc agtcgcatcc caaacggatg ttacgtcata aagccatgat tcagtgtgcc
      481 cgtctggcct tcggatttgc tggtatctat gacaaggatg aagccgagcg cattgtcgaa
      541 aatactgcat acactgcaga acgtcagccg gaacgcgaca tcactccggt taacgatgaa
      601 accatgcagg agattaacac tctgctgatc gccctggata aaacatggga tgacgactta
      661 ttgccgctct gttcccagat atttcgccgc gacattcgtg catcgtcaga actgacacag
      721 gccgaagcag taaaagctct tggattcctg aaacagaaag ccgcagagca gaaggtggca
      781 gcatgaggat cccatggtac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtt
//
