LOCUS       sequence-100979-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-12a
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taaggtaccg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcatca
       61 tcatcatcat cactgactga aaagaacggg aagatttatg aagaaaggtt ttatgttgtt
      121 tactttgtta gcggcgtttt caggctttgc tcaggctgat gacgcggcaa ttcaacaaac
      181 gttagccaaa atgggcatca aaagcagcga tattcagccc gcgcctgtag ctggcatgaa
      241 gacagttctg actaacagcg gcgtgttgta catcaccgat gatggtaaac atatcattca
      301 ggggccaatg tatgacgtta gtggcacggc tccggtcaat gtcaccaata agatgctgtt
      361 aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca
      421 cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc atgagcaaat
      481 ggcagactac aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc gccaggggct
      541 ggacagcgat gcagagaaag aaatgaaagc tatctggtgt gcgaaagata aaaacaaagc
      601 gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agttgcgacg tggatattgc
      661 cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag ttgtgctgag
      721 caatggcaca cttgttccgg gttaccagcc gccg
//
