LOCUS       sequence-100982-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-17a
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taccggatcc gaattcgagc tccgtcgaca agcttgcggc ccactcgagc accaccacca
       61 ccaccactga aaagtttata ccttacctgg aacaaatggt tgaaacatac gaggctaata
      121 tcggcttatt aggaatagtc cctgtactaa taaaatcagg tggatcagtt gatcagtata
      181 ttttggacga agctcggaaa gaatttggag atgacttgct taattccaca attaaattaa
      241 gggaaagaat aaagcgattt gatgttcaag gaatcacgga agaagatact catgataaag
      301 aagctctaaa actattcaat aaccttacaa tggaattgat cgaaagggtg gaaggttaat
      361 ggtacgaaaa ttaggggatc tacctagaaa gccacaaggc gataggtcaa gcttaaagaa
      421 cccttacatg gatcttacag attctgaaag taaagaaaca acagaggtta aacaaacaga
      481 accaaaaaga aaaaaagcat tgttgaaaac aatgaaagtt gatgtttcaa tccataataa
      541 gattaaatcg ctgcacgaaa ttctggcagc atccgaaggg aattcatatt acttagagga
      601 tactattgag agagctattg ataagatggt tgagacatta cctgagagcc aaaaaacttt
      661 ttatgaatat gaattaaaaa aaagaaccaa caaaggctga gacagactcc aaacgagtct
      721 g
//
