LOCUS       sequence-100984-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-18a
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taccggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc
       61 accaccactg aaaagtttat accttacctg gaacaaatgg ttgaaacata cgaggctaat
      121 atcggcttat taggaatagt ccctgtacta ataaaatcag gtggatcagt tgatcagtat
      181 attttggacg aagctcggaa agaatttgga gatgacttgc ttaattccac aattaaatta
      241 agggaaagaa taaagcgatt tgatgttcaa ggaatcacgg aagaagatac tcatgataaa
      301 gaagctctaa aactattcaa taaccttaca atggaattga tcgaaagggt ggaaggttaa
      361 tggtacgaaa attaggggat ctacctagaa agccacaagg cgataggtca agcttaaaga
      421 acccttacat ggatcttaca gattctgaaa gtaaagaaac aacagaggtt aaacaaacag
      481 aaccaaaaag aaaaaaagca ttgttgaaaa caatgaaagt tgatgtttca atccataata
      541 agattaaatc gctgcacgaa attctggcag catccgaagg gaattcatat tacttagagg
      601 atactattga gagagctatt gataagatgg ttgagacatt acctgagagc caaaaaactt
      661 tttatgaata tgaattaaaa aaaagaacca acaaaggctg agacagactc caaacgagtc
      721 tg
//
