LOCUS       sequence-100986-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-15a
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..105
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             121..138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      217..463
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 taccggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag ccgcggcggc
       61 cgccagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag cgccgtcgac
      121 catcatcatc atcatcattg agttttagcc ttagacatga ctgttcctca gttcaagttg
      181 ggcacttacg agaagaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt
      241 tgcctgagag atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag
      301 gatttttttt gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc
      361 agctgatgaa tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt
      421 ggtatttccc actcctcttc agagtacaga agattaagtg agaccttcgt ttgtgcggat
      481 cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc
      541 ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc
      601 tctttcttcc tctagggtgt cgttaattac ccgtact
//
