LOCUS       sequence-100989-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-1a
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(121..139)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      135..182
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      219..674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(306..325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     516..537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taccggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc
       61 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      121 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      181 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      241 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      301 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      361 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      421 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      481 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      541 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      601 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
      661 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      721 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttag
//
