LOCUS       sequence-100994-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Jin J, Ang XL, Shirogane T, Wade Harper J
  TITLE     Identification of substrates for F-box proteins.
  JOURNAL   Methods Enzymol. 2005;399:287-309. doi: 
            10.1016/S0076-6879(05)99020-4.
  PUBMED    16338364
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2005"; volume: "399:287-309. doi"; pages: " 
            10.1016/S0076-6879(05)99020-4"
COMMENT     Sequence Label: pcDNA3-DN-hCUL3-FLAG
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatct gccaccatgt
      121 cgaatctgag caaaggcacg ggcagccgga aggacaccaa gatgcggatc cgggcctttc
      181 cgatgaccat ggatgaaaaa tatgtaaaca gcatttggga ccttctgaaa aatgcaattc
      241 aagaaatcca gcgtaagaat aacagtggtc ttagttttga ggagctctat agaaatgcat
      301 atacaatggt tttgcataaa catggagaaa agctctacac tggactaaga gaagttgtta
      361 ccgaacatct cataaataag gtgcgagaag atgtactaaa ttcattgaat aacaactttc
      421 ttcaaacgct aaatcaagct tggaatgatc atcaaacagc tatggtgatg attagagaca
      481 tactaatgta catggaccgt gtgtatgtac aacaaaataa tgtggagaac gtctacaatt
      541 tgggattaat tatttttcga gatcaagttg tacgttatgg gtgtattagg gatcatctac
      601 ggcaaactct attggatatg attgcaagag agcggaaagg agaagtcgta gacagaggcg
      661 caataagaaa tgcttgccag atgttaatga ttttaggtct cgaaggaaga tcagtctatg
      721 aagaagattt tgaggctcct tttttggaaa tgtctgcaga attttttcag atggaaagcc
      781 agaaattttt agcagaaaat agtgcttcag tatatataaa gaaagtagaa gctagaatta
      841 atgaagaaat agaacgagtg atgcactgcc ttgacaaatc aacggaagaa ccaattgtaa
      901 aggtggttga aagggaactc atttccaagc acatgaagac tatagtagaa atggagaatt
      961 ctgggctagt acatatgttg aaaaatggaa agacagaaga ccttggttgc atgtacaagt
     1021 tatttagtcg tgtgccaaat ggtttgaaaa caatgtgtga gtgtatgagt tcctatttga
     1081 gggagcaagg taaagctctt g
//
