LOCUS       sequence-100995-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Jin J, Ang XL, Shirogane T, Wade Harper J
  TITLE     Identification of substrates for F-box proteins.
  JOURNAL   Methods Enzymol. 2005;399:287-309. doi: 
            10.1016/S0076-6879(05)99020-4.
  PUBMED    16338364
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2005"; volume: "399:287-309. doi"; pages: " 
            10.1016/S0076-6879(05)99020-4"
COMMENT     Sequence Label: pcDNA3-DN-hCUL3-FLAG
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        106..124
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     106..123
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(155..178)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcgagct ctagcattta ggtgacacta
      121 tagaataggg ccctctagat gcatgctcga gtcacttgtc atcgtcatcc ttgtagtcaa
      181 ggaccattgc tttatccaat attgtttcta cttcttgttc tgttagccct ttgactccct
      241 ttttcagctt atcatcaata aataatgaga ggtattcagg agacctggag ttgaggttga
      301 gaaaatactc aaagtcaccc gcaatagttt gtttaaagag acggtcattg ttgaatgatt
      361 ccaggaggaa gcgatcgaac ctactcttca gatccaataa gccctggata tagtcaacag
      421 gattctttcc ttctccttct tcagaaacaa gagctttacc ttgctccctc aaataggaac
      481 tcatacactc acacattgtt ttcaaaccat ttggcacacg actaaataac ttgtacatgc
      541 aaccaaggtc ttctgtcttt ccatttttca acatatgtac tagcccagaa ttctccattt
      601 ctactatagt cttcatgtgc ttggaaatga gttccctttc aaccaccttt acaattggtt
      661 cttccgttga tttgtcaagg cagtgcatca ctcgttctat ttcttcatta attctagctt
      721 ctactttctt tatatatact gaagcactat tttctgctaa aaatttctgg ctttccatct
      781 gaaaaaattc tgcagacatt tccaaaaaag gagcctcaaa atcttcttca tagactgatc
      841 ttccttcgag acctaaaatc attaacatct ggcaagcatt tcttattgcg cctctgtcta
      901 cgacttctcc tttccgctct cttgcaatca tatccaatag agtttgccgt agatgatccc
      961 taatacaccc ataacgtaca acttgatctc gaaaaataat taatcccaaa ttgtagacgt
     1021 tctccacatt attttgttgt acatacacac ggtccatgta cattagtatg tctctaatca
     1081 tcaccatagc tgtttga
//
