LOCUS       sequence-100996-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, 
            Cantley LC
  TITLE     The tumor suppressor LKB1 kinase directly activates AMP-activated 
            kinase and regulates apoptosis in response to energy stress.
  JOURNAL   Proc Natl Acad Sci U S A 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 
            25.
  PUBMED    14985505
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2004-03-9"; volume: "101(10)"; pages: 
            "3329-35. Epub 2004 Feb 25"
COMMENT     Sequence Label: pBABE-FLAG-KD LKB1
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..48
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggatctaa gcttagccac catggactac aaagacgatg acgataaagc aagggatcca
       61 ctagtaacgg ccgccagtgt gctggaattc tccagcatgg aggtggtgga cccgcagcag
      121 ctgggcatgt tcacggaggg cgagctgatg tcggtgggta tggacacgtt catccaccgc
      181 atcgactcca ccgaggtcat ctaccagccg cgccgcaagc gggccaagct catcggcaag
      241 tacctgatgg gggacctgct gggggaaggc tcttacggca aggtgaagga ggtgctggac
      301 tcggagacgc tgtgcaggag ggccgtcatc atcctcaaga agaagaagtt gcgaaggatc
      361 cccaacgggg aggccaacgt gaagaaggaa attcaactac tgaggaggtt acggcacaaa
      421 aatgtcatcc agctggtgga tgtgttatac aacgaagaga agcagaaaat gtatatggtg
      481 atggagtact gcgtgtgtgg catgcaggaa atgctggaca gcgtgccgga gaagcgtttc
      541 ccagtgtgcc aggcccacgg gtacttctgt cagctgattg acggcctgga gtacctgcat
      601 agccagggca ttgtgcacaa ggacatcaag ccggggaacc tgctgctcac caccggtggc
      661 accctcaaaa tctccgacct gggcgtggcc gaggcactgc acccgttcgc ggcggacgac
      721 acctgccgga ccagccaggg ctccccggct ttccagccgc ccgagattgc caacggcctg
      781 gacaccttct ccggcttcaa ggtggacatc tggtcggctg gggtcaccct ctacaacatc
      841 accacgggtc tgtacccctt cgaaggggac aacatctaca agttgtttga gaacatcggg
      901 aaggggagct acgccatccc gggcgactgt ggccccccgc tctctgacct gctgaaaggg
      961 atgcttgagt acgaaccggc caagaggttc tccatccggc agatccggca gcacagctgg
     1021 ttccggaaga aacatcctcc ggctgaagca ccagtgccca tcccaccgag cccagacacc
     1081 aaggaccggt ggcgca
//
