LOCUS       sequence-100999-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX2
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..147
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     387..408
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
       61 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      121 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      181 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
      241 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      301 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      361 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      421 cccggtgaac agctcctcgc ccttgctcac catagaccca ccacccccgg atccaatctc
      481 caggtcatca ggccgaggcc tgctgctggc gcggctgctg gctctgctgg aaggtcgttg
      541 gtccacgatg gctaatggct ggagttcatg tccagcagca acttttttgg cattctggtt
      601 gtcgtcgggg aaatcgaacg gctgggcgtg cgagttggag atggtgcttc cggcctgccc
      661 catgcgattt tgctctgcgc tgtagttcgc ccagttttgc tcgctagctt gcttgttgta
      721 attgcggcac gaggaattgt ttctgtcacc agtaaccagc ttgtacccag gaggagacat
      781 aggcgagagt ggagccgttg gtgaggagca gccattgaag taggcgtatt ttggagatcc
      841 gcagtctttt gatgggctca gtgggccagt ggtggcgtgg taaggatcgc ttcttccctt
      901 cacgcgatcc ttaacgccct tgaagaagac gtagaagagc tcaatgatgt tcaaagcgag
      961 agacaccaag gacaccacca gcatgaagat gatgaagatg gttttctccg tgggacgtga
     1021 gaggaagc
//
