LOCUS       sequence-101000-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX2
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     315..336
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
       61 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      121 atggccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      181 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      241 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      301 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      361 atggacgagc tgtacaaggg gcgcgccaag ggctcgggct cgacctcggg ctcgggcgga
      421 aagtcttacc caactgtgag tgctgattac caggacgccg ttgagaaggc gaagaagaag
      481 ctcagaggct tcatcgctga gaagagatgc gctcctctaa tgctccgttt ggcattccac
      541 tctgctggaa cctttgacaa gggcacgaag accggtggac ccttcggaac catcaagcac
      601 cctgccgaac tggctcacag cgctaacaac ggtcttgaca tcgctgttag gcttttggag
      661 ccactcaagg cggagttccc tattttgagc tacgccgatt tctaccagtt ggctggcgtt
      721 gttgccgttg aggtcacggg tggacctaag gttccattcc accctggaag agaggacaag
      781 cctgagccac caccagaggg tcgcttgccc gatcccacta agggttctga ccatttgaga
      841 gatgtgtttg gcaaagctat ggggcttact gaccaagata tcgttgctct atctgggggt
      901 cacactattg gagctgcaca caaggagcgt tctggatttg agggtccctg gacctctaat
      961 cctcttattt tcgacaactc atacttcacg gagttgttga gtggtgagaa ggaaggtctc
     1021 cttcagctac cttctgacaa ggctcttttg
//
