LOCUS       sequence-101001-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX2
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    123..347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     336..353
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
       61 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
      121 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
      181 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      241 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      301 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      361 gcgggtttaa acgggccctc tagactcgag ttaggcatca gcaaacccaa gctcggaaag
      421 cttttggtga gcctcagcgt aatcagcaaa gaaggcatct tcgtccgctg catatttgtc
      481 aacgagaggg cggaatacag ggtcagacaa aagagccttg tcagaaggta gctgaaggag
      541 accttccttc tcaccactca acaactccgt gaagtatgag ttgtcgaaaa taagaggatt
      601 agaggtccag ggaccctcaa atccagaacg ctccttgtgt gcagctccaa tagtgtgacc
      661 cccagataga gcaacgatat cttggtcagt aagccccata gctttgccaa acacatctct
      721 caaatggtca gaacccttag tgggatcggg caagcgaccc tctggtggtg gctcaggctt
      781 gtcctctctt ccagggtgga atggaacctt aggtccaccc gtgacctcaa cggcaacaac
      841 gccagccaac tggtagaaat cggcgtagct caaaataggg aactccgcct tgagtggctc
      901 caaaagccta acagcgatgt caagaccgtt gttagcgctg tgagccagtt cggcagggtg
      961 cttgatggtt ccgaagggtc caccggtctt cgtgcccttg tcaaaggttc cagcagagtg
     1021 gaatgccaaa cggagcatta gaggagcgca tctct
//
