LOCUS       sequence-101013-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-4b
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(127..145)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      141..188
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      225..680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(312..331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     522..543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtaaggtacc ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc
       61 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      121 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      181 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      241 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      301 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      361 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      421 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      481 ggtttttcgc catttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      541 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      601 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      661 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      721 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt cttag
//
