LOCUS       sequence-101030-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-4a-Strep
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..80
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(160..178)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      174..221
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      258..713
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(345..364)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     555..576
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtaaggtacc ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagtgga
       61 gccatccaca attcgaaaag taaactagtc accaccacca ccaccactga gatccggctg
      121 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      181 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      241 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      301 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      361 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      421 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      481 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      541 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
      601 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      661 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
      721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      781 atgtatccgc tcatgaatta attcttag
//
