LOCUS       sequence-101031-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-7a-Strep
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..79
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(159..177)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      173..220
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      257..712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(344..363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     554..575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtaaggtacc ggatccgaat tcgagctccg tcgacaagct tgcggcccac tcgagtggag
       61 ccatccacaa ttcgaaaagt aaactagtca ccaccaccac caccactgag atccggctgc
      121 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      181 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      241 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      301 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      361 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      421 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      481 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      541 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      601 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
      661 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      721 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      781 tgtatccgct catgaattaa ttcttag
//
