LOCUS       sequence-101034-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AMPK biosensor targeted to the plasma membrane via Lyn
            kinase targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: PM-ABKAR
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(135..137,138..140,141..851)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(180..201)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(441..460)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     788..809
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttgcggccg ccaccatggg ctgcatcaag agcaagcgca
      121 aggacaagga tcccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      181 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      241 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      301 tgccctggcc caccctcgtg accaccctga gctggggcgt gcagtgcttc gcccgctacc
      361 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      421 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      481 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      541 acatcctggg gcacaagctg gagtacaacg ccatccacgg caacgtctat atcaccgccg
      601 acaagcagaa gaacggcatc aaggccaact tcggcctcaa ctgcaacatc gaggacggca
      661 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      721 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      781 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      841 agctgtacaa gcgcatgcat aag
//
