LOCUS       sequence-101038-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AMPK biosensor targeted to the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: ER-ABKAR
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(260..281)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(521..540)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc ccttgcagct tccagttgtc aggaagagaa
      121 ggctcccatg gaccctgtgg tggtgctggg gctctgtctc tcctgtttgc ttctcctttc
      181 actctggaaa cagagctatg ggggagggga tcccatggtg agcaagggcg aggagctgtt
      241 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      301 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      361 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gctggggcgt
      421 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      481 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      541 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      601 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatccacgg
      661 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcggcctcaa
      721 ctgcaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      781 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agca
//
