LOCUS       sequence-101043-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AMPK biosensor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: Cyto-ABKAR
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..761
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     161..182
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(288..309)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(549..568)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             768..794
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 acctcggcac cggcggcagc gagctcatgg gcggcgtgca gctcgccgac cactaccagc
       61 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
      121 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      181 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggaggt accggtggat
      241 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      301 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      361 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
      421 ccctcgtgac caccctgggc tacggccttc agtgcttcgc ccgctacccc gaccacatga
      481 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      541 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      601 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      661 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
      721 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggaattcctg ccccccctgg
      781 agcgcctgac cctgtaatct aga
//
