LOCUS       sequence-101045-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of nuclear AMPK biosensor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: Nuc-ABKAR
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..834
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     234..255
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(361..382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(622..641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             841..861
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttgatcgct ctgcaggtaa gccaggcagc ggcgagggca gcaccaagat gcgtcgtgtc
       61 gccaccctgg tggacctcgg caccggcggc agcgagctca tgggcggcgt gcagctcgcc
      121 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      181 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      241 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga
      301 ggtaccggtg gatctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      361 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      421 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
      481 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc ttcagtgctt cgcccgctac
      541 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      601 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      661 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      721 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
      781 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggaattc
      841 cccaaaaaga agagaaaggt ggaagacgca t
//
