LOCUS       sequence-101047-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human FK506 binding protein 1A (FKBP1A) tagged with 
            EYFP and AIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: YF-AIP
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..259
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     196..217
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             272..592
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
       61 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      121 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
      181 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      241 catggacgag ctgtacaaga gtgctggtgg tggagtgcag gtggaaacca tctccccagg
      301 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct
      361 tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct
      421 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
      481 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat
      541 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaagtgctgg
      601 tggtagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtcctcgagc
      661 tcaagcttcg aattcaggat caggagaggg atcaaccaag atgagaagag tggctactct
      721 ggtggacctt ggaaccggag gatcagagct ctgactgca
//
