LOCUS       sequence-101066-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human FK506 binding protein 1A (FKBP1A) tagged with 
            mCherry, Tom20, and AIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: Tom20-mChF-AIP
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             156..863
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(301..319)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     569..588
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggtgaattcg ccaccatggt gggtcggaac agcgccatcg
       61 ccgcgggcgt gtgcggtgcc ctcttcatag ggtactgcat ctactttgac cgcaaaagac
      121 gaagtgaccc caacttcgga tccggagcaa gtggaatggt gagcaagggc gaggaggata
      181 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      241 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      301 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      361 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      421 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      481 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      541 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      601 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      661 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      721 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
      781 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      841 gcggcatgga cgaactgtac aagagtgctg gtggtggagt gcaggtggaa acca
//
