LOCUS       sequence-101069-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human FK506 binding protein 1A (FKBP1A) tagged with 
            mCherry, Tom20, and AIP with TA mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: Tom20-mChF-AIP(TA)
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(309..327)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     577..596
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tgaattcgcc accatggtgg gtcggaacag
       61 cgccatcgcc gcgggcgtgt gcggtgccct cttcataggg tactgcatct actttgaccg
      121 caaaagacga agtgacccca acttcggatc cggagcaagt ggaatggtga gcaagggcga
      181 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      241 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      301 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      361 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      421 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      481 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      541 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      601 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      661 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      721 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      781 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg a
//
