LOCUS       sequence-101076-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting construct for human NANOS3 locus, introducing a 
            P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette 
            that can be excised..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, 
            Dietmann S, Hanna JH, Surani MA
  TITLE     SOX17 is a critical specifier of human primordial germ cell fate.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. 
            Epub 2014 Dec 24.
  PUBMED    25543152
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):253-68. doi"; pages: " 
            10.1016/j.cell.2014.12.013. Epub 2014 Dec 24"
COMMENT     Sequence Label: Human NANOS3-P2A-mCherry knock-in targeting
            co...
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    266..299
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(316..349)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(405..428)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
       61 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      121 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      181 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      241 aggctagctc cgggtctagc cgcggataac ttcgtataat gtatgctata cgaagttatg
      301 aattctagga tcgatgaagt tcctatactt tctagagaat aggaacttcg gatcccggcg
      361 cgccctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      421 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      481 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      541 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      601 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      661 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      721 tgggaagggg tgggtccggg ggcgggctca
//
