LOCUS       sequence-101081-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) 
            tagged with mCherry and AIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: AIP(WT)-mChF-giantin
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..821
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(259..277)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     527..546
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcaccgcc atgggatcag gagagggatc aaccaagatg agaagagtgg
       61 ctactctggt ggaccttgga accggaggat cagagctcat accggtcgcc accatggtaa
      121 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      181 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      241 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      301 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      361 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      421 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      481 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      541 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      601 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      661 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      721 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      781 agggccgcca ctccaccggc ggcatggacg agctgtacaa gagtgctggt ggtggagtgc
      841 a
//
