LOCUS       sequence-101088-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a guide RNA (gRNA) to target human IRF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: gRNA-hIRF-1 #12/pSIR
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        673..921
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     673..693
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     844..863
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgcca aacctaaacc tcaagttctt
       61 tctgacagtg gggggccgct catcgaccta cttacagaag accccccgcc ttatagggac
      121 ccaagaccac ccccttccga cagggacgga aatggtggag aagcgacccc tgcgggagag
      181 gcaccggacc cctccccaat ggcatctcgc ctacgtggga gacgggagcc ccctgtggcc
      241 gactccacta cctcgcaggc attccccctc cgcgcaggag gaaacggaca gcttcaatac
      301 tggccgttct cctcttctga cctttacaac tggaaaaata ataacccttc tttttctgaa
      361 gatccaggta aactgacagc tctgatcgag tctgtcctca tcacccatca gcccacctgg
      421 gacgactgtc agcagctgtt ggggactctg ctgaccggag aagaaaaaca acgggtgctc
      481 ttagaggcta gaaaggcggt gcggggcgat gatgggcgcc ccactcaact gcccaatgaa
      541 gtcgatgccg cttttcccct cgagcggccg ccagtgtgat ggatatctgc agaattcgcc
      601 ctttgtacaa aaaagcaggc tttaaaggaa ccaattcagt cgactggatc cggtaccaag
      661 gtcgggcagg aagagggcct atttcccatg attccttcat atttgcatat acgatacaag
      721 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
      781 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      841 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      901 ttgtggaaag gacgaaacac cgcgggg
//
