LOCUS       sequence-101090-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme
            in E. coli. Also known as pAt9SK..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Cunningham FX Jr, Lee H, Gantt E
  TITLE     Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon 
            merolae.
  JOURNAL   Eukaryot Cell. 2007 Mar;6(3):533-45. Epub 2006 Nov 3.
  PUBMED    17085635
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2007-03"; volume: "6(3)"; pages: "533-45. Epub 2006 
            Nov 3"
COMMENT     Sequence Label: pAtLCYbSK
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     107..123
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     108..124
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
       61 gcgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggt
      121 atcgataagc ttggatcctc tagagcggcc gccctttttt tttttttttt tttttttgtg
      181 ttcaaaatcc actcttttat cttattacat tttgcctcta gttttggatt tacaagagtt
      241 ggtgaaacac aatgcagcac aaagtattaa ttttaatgaa ctagtagtaa caatttgatt
      301 tcacaaggat tcaggttatg atctgtggtt tatacacaat tatccaacga cttgcaatgc
      361 ggatatacta ctggtcaaga accaaagaac agatgtactt atatgtctaa gtttctggtc
      421 cttagtctct atcttgtacc aaattgttga tcatcttagc aagaggaaca gtcccctttg
      481 tcatgatctc caatcttgag gtattggaag cgtgtgagaa gagcgacaac ccgaagacca
      541 acagttccgg gagaaacagc ctggaagaca agaatccgtg ccagtaatga ggttgcagat
      601 caaagaatgc atcaaagaac cttctagtag cgtctaaatc gagtttcagc agaatatcca
      661 ttccaaaaca gaagaactcc ctctgtctac gccgttcgat aggccacaag tctctccaaa
      721 cctcagcaga
//
