LOCUS       sequence-101099-         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express C-terminal Ubl4A K48R, for making the stable cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pcDNA 5/FR/TO-Ubl4A K48R
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             511..540
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             559..582
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aagcttgccg ccgcgatcgc catgcagctg acggtgaagg cgctgcaggg ccgcgagtgc
       61 agcctgcagg tgccagagga cgagctggtg tccacgctga agcagctggt ctccgagaag
      121 ctgaacgtcc cagtgcgcca gcagcggctg ctgttcaagg gcagggccct ggcagatggg
      181 aaacgactct cggattatag catcgggccc aactccaagc tcaacctagt ggtcaaaccc
      241 ctggagaagg tgctactaga agaaggcgag gcccagaggc tggccgactc cccacccccg
      301 caggtctggc agctgatctc caaagtcttg gcccgccact tcagtgcggc agatgccagc
      361 agggtcctgg aacagctaca gagggattac gagaggtccc tgagtcgcct gacgctggac
      421 gacatcgaac ggttggccag ccgcttcctg caccctgaag tgactgagac aatggagaag
      481 ggcttctcca aaacgcgtac gcggccgctc gagcagaaac tcatctcaga agaggatctg
      541 gcagcaaatg atatcctgga ttacaaggat gacgacgata agtagggatc c
//
